Green Synthesis of Silver Nanoparticles:Eco-Friendly and Antibacterial

Document Type : Research Paper

Authors

1 Department of Plant Breeding, Faculty of Agricultureal, Shahid Bahonar University of Kerman, Young Researchers and Elite Club, Ahvaz Branch, Islamic Azad University, Ahvaz, I. R. Iran

2 Department of Biotechnology, Faculty of Agricultureal, Shahid Bahonar University, Kerman, I. R. Iran

3 Department of Engineer Chemistry, Faculty of Engineering, Shahid Bahonar University, Kerman, I. R. Iran

Abstract

Nano world daily. In this paper the Lens culinaris seed extract was assessed for thegreen synthesis of silvernanoparticlesat temperature of 25◦C. The nanoparticles were characterized using Inductively CoupledPlasma spectrometry (ICP), X-ray diffraction (XRD) and Transmission electron microscopy (TEM). Thenthe antimicrobial activities of these nanoparticles were investigated. P. aeruginosa and S aureus were used as representatives of Gram-negative and Gram-positive bacteria, respectively. Percentage conversion of metal ion to metal nanoparticles were more than 95% after 60 days of the reaction. XRD was used to confirm the crystalline nature of the particles. Silver nanoparticles were mostly spherical with range in size from 5-25 nm. Ag nanoparticles synthesized with extract were exhibited a strong antibacterial activity against bothP.aeruginosa and S.aureus.This process is completely green and eco-friendly compatible.

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